Animal Cell Culture and Technology (THE BASICS (Garland by Michael Butler

By Michael Butler

This booklet is a superb source for college students and starting clinical reaseraches. it's a solid resource of uncomplicated tools and methods for winning laboratory cellphone tradition strategies. many of the present telephone tradition tools are lined yet this assurance isn't very precise. back, very good for newcomers.

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The polystyrene used to make most domestic plastic containers is unsuitable as a surface for cell attachment because it is hydrophobic and has no charge. The ‘tissue culture-grade’ plasticware available from commercial sources is generally made of polystyrene, which is treated to allow a negative surface charge (Maroudas, 1977). This provides a surface chemistry that is hydrophilic, wetable and negatively charged. The charge may be provided by short exposure of the polystyrene to sulfuric acid to allow sulfonation of the surface.

When it is not possible to have an independent sterile handling laboratory, it is at least necessary to ensure that the sterile handling area of a larger laboratory is positioned in a region where there is minimal Basic equipment and laboratory design 31 movement of people. The area used for non-sterile work such as the wash-up facility and disposal of used culture flasks should be positioned at the other side of the laboratory from the clean area. 1. This is a self-contained laboratory suitable for use by two or three people.

Routine maintenance requires regular cleaning and disinfection of the inner walls and trays of the chamber, which are made of stainless steel. g. Cytoperm from Heraeus) an automated disinfection routine involves the introduction of hot air at 180°C. For many laboratories double cabinet incubators are useful. One chamber is stacked above the other but they are independently regulated. 3 shows two incubator chambers stacked one on top of the other. The CO2 is supplied from the cylinder seen on the left.

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