Chemistry of Protein and Nucleic Acid Cross-Linking and by Shan S. Wong

By Shan S. Wong

Since the ebook of the 1st version of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, significantly multifunctional cross-linkers, were built and synthesized. The crowning glory of the human genome undertaking has opened a brand new region for learning nucleic acid and protein interactions utilizing nucleic acid cross-linking reagents, and advances have additionally been made within the quarter of biosensors and microarray biochips for the detection and research of genes, proteins, and carbohydrates. moreover, advancements in actual ideas with unheard of sensitivity and backbone have facilitated the research of cross-linked items.

Updated to mirror the advances of the twenty first century, this booklet offers:

  • An evaluate of the chemical ideas underlying the techniques of cross-linking and conjugation
  • A thorough checklist of cross-linking reagents released within the literature because the first version, overlaying monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers
  • Reviews of using those reagents in learning protein tertiary buildings, geometric preparations of subunits inside advanced proteins and nucleic acids, near-neighbor research, protein-to-protein or ligand–receptor interactions, and conformational alterations of biomolecules
  • Discusses the applying of immunoconjugation for immunoassays, immunotoxins for certain remedy, microarray know-how for research of varied biomolecules, and reliable nation chemistry for immobilizations

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Additional info for Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation, Second Edition

Example text

90 It has been estimated that about 1000 nucleotides are depurinated in a typical mammalian cell each day; however, DNA repair mechanisms have evolved to help mitigate this problem. Interestingly, the toxic protein ricin catalyzes adenosine depurination of the 28S eukaryotic ribosomal RNA at position 4256, which results in total loss of the ribosome’s ability to synthesize proteins, thus leading to cell death. Perhaps the most important chemical reaction of nucleic acids for cross-linking is alkylation.

Molecular mechanisms in nucleic acid photochemistry. I. Sensitized photochemical splitting of thymine dimer, J. Am. Chem. , 88, 6113, 1966. 85. Pashev, I. , Dimitrov, S. , Crosslinking proteins to nucleic acids by ultraviolet laser irradiation, Trends Biochem. , 16, 323, 1991. 86. Nikogosyan, D. , Two-quantum UV photochemistry of nucleic acids: Comparison with conventional low-intensity UV photochemistry and radiation chemistry, Int. J. Radiat. , 57, 233, 1990. 34 Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation 87.

Hershey, J. W. , and Pierce, L. , Methyl 4-mercaptobutyrimidate as cleavable crosslink reagent and its application to the Escherichia coli 30s ribosome, Biochemistry, 12, 3266, 1973. 48. Kenny, J. , and Traut, R. , Cross-linking studies on the 50S ribosomal subunit of Escherichia coli with methyl 4-mcrcaptobutyrimidate, J. Biol. , 250, 9434, 1975. 49. King, T. , Preparation of protein conjugates via intermolecular disulfide bond formation, Biochemistry, 17, 1499, 1978. 50. , Lambert, J. , Pierce, L.

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