Chiral Separation Techniques: A Practical Approach by Ganapathy Subramanian

By Ganapathy Subramanian

Completely revised, with both solely new or thoroughly up-to-date contents, it is a useful guide for the small and large-scale instruction of enantiomerically natural items. the result's an essential source for assembly the top purity criteria within the manufacture of chiral prescribed drugs, nutrition ingredients and comparable compounds. the entire ways lined listed below are hugely appropriate to fashionable production and quality controls schemes within the pharmaceutical and biotech industries, addressing the more and more very important factor of drug protection in view of more durable regulatory criteria world wide.

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3 Properties of Polysaccharide CSPs As discussed above, polysaccharide CSPs are fairly stable in the normal- and reversed-phase modes. They have high chiral recognition selectivities, efficiencies and capabilities for a variety of racemates.

Pharmazie 1998, 53, 863–865. , Armstrong D. W. J. Chromatogr. A 2001, 926, 229–238. , Armstrong D. W. J. Chromatogr. A 2004, 1057, 229–235. Beesley T. E. Amino Acid and Peptide Chiral Separation Handbook, 1st edn. Advanced Separation Technologies, 2005. -H. Chirality 2001, 13, 231–235. , Pacakova V. J. Chromatogr. A 1999, 838, 121–129. Desai M. , Armstrong D. W. J. Mass Spectrom. A 2004, 39, 177–187. , Dreux M. J. Chromatogr. A 2001, 913, 331–340. , Armstrong D. W. J. Chromatogr. A 2004, 1053, 89–99.

15 Separation of bifunctional amino acids using a teicoplanin column. 8 mL min–1; detection, ELSD. 7 Amino Acid and Peptide Analysis Fig. 16 Separation of six peptides of the enkephalin family (neutral peptides) using a teicoplanin column. Single amino acid analogs are peaks 2 and 3, peaks 4 and 5 and peaks 5 and 6. Chiral amino acid analogs are peaks 1 and 3 and peaks 3 and 6. 5 mL min–1; UV detection at 230 nm. 2 and 3, peaks 4 and 5 and peaks 5 and 6) and also single chiral amino acid isoforms (peaks 1 and 3 and peaks 3 and 6).

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